Chitooligosaccharide-catechin conjugate loaded liposome using different stabilising agents: characteristics, stability, and bioactivities

Abstract

Aim

To determine the optimum condition for preparing chitooligosaccharide-catechin conjugate (COS-CAT) liposomes using different stabilising agents.

Methods

COS-CAT liposomes (0.1–1%, w/v) were prepared using soy phosphatidylcholine (SPC) (50–200 mM) and glycerol or cholesterol (25–100 mg). Encapsulation efficiency (EE), loading capacity (LC), physicochemical characteristics, FTIR spectra, thermal stability, and structure of COS-CAT liposomes were assessed.

Results

COS-CAT loaded liposome stabilised by cholesterol (COS-CAT-CHO) showed higher stability as shown by the highest EE (76.81%) and LC (4.57%) and the lowest zeta potential (ZP) (−76.51 mV), polydispersity index (PDI) (0.2674) and releasing efficiency (RE) (53.54%) (p < 0.05). COS-CAT-CHO showed the highest retention and relative remaining bioactivities of COS-CAT under various conditions (p < 0.05). FTIR spectra revealed the interaction between the choline group of SPC and -OH groups of COS-CAT. Phase transition temperature of COS-CAT-CHO was shifted to 184 °C, which was higher than others (p < 0.05).

Conclusion

SPC and cholesterol-based liposome could be used as a promising vesicle for maintaining bioactivities of COS-CAT.

Keywords:

• Chitooligosaccharide
• catechin
• conjugate
• liposome
• antioxidant
• bioactivity
• in vitro digestion
• stability

Acknowledgements

International Research Network (IRN) program from the National Research Council of Thailand (NRCT) was acknowledged. Chair professor grant (P-20-52297) from National Science and Technology Development Agency (NSTDA), Thailand and Prachayacharn Grant awarded by Prince of Songkla University, Hat Yai, Thailand (Grant no. AGR6502111N) was also acknowledged.

Disclosure statement

The authors report there are no competing interests to declare.

Data availability statement

The research data were not shared.