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Abstract
A four-channel frequency division multiplexed fluorescent microscope (FDMFM) modulated by holographic polymer dispersed liquid crystal (H-PDLC) grating array is presented in this paper. The four-channel H-PDLC grating array is developed and employed to generate frequency modulation. Fluorescent signals at four different locations on a rat neural cell have been successfully modulated with four different frequencies, collected by a single high-sensitivity photodetector, such as photomultiplier tubes, and distinguished by taking the Fourier transform of the detected sum signal. The experimental and simulation results agree well. The new system offers the advantages of (1) multiple channel modulation capability, (2) no mechanical noise and (3) flexible and controllable modulation profile (e.g. square wave, triangular wave, etc.). Thus this four-channel FDMFM system can be very helpful for studying fast-changing transient events in biological samples.
Acknowledgement
The authors acknowledge Prof. Xiang Zhenghua from the second military medical university for providing the experimental specimens.
Funding
This work was supported by the Young Scientists Fund of the National Natural Science Foundation of China [grant number 60801041]; the Key Research Project from Shanghai Education Committee [grant number 14ZZ138]; the Leading Academic Discipline Project of Shanghai Municipal Government [grant number S30502]; Graduate’s Innovation Fund of University of Shanghai for Science and Technology [grant number JWCXSL1202]; and Development Fund for Shanghai Talents [grant number 2012026].