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Quadrupolar splittings in the 2H NMR spectrum obtained from a sample with natural abundance 2H isotope are shown to be considerably different from those obtained from specifically deuteriated samples in an example of 4'-propyl-4-(3,4-difluorophenyl)bicyclohexane. The result indicates that the molecular ordering may be altered significantly by selective deuteriation, and shows the importance of natural abundance 2H measurement in liquid crystal studies.
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