Abstract
Conidial forms of Fonsecaea pedrosoi, grown under conditions where melanin was or was not synthesized, were allowed to interact with normal and cytochalasin treated macrophages. Melanin-free conidia were more infective to the macrophages. Treatment of macrophages with either cytochalasin B or D before the interaction decreased, but did not totally prevent their infection by the fungi. This inhibitory effect was higher (approximately 90%) if F. pedrosoi was grown under conditions where melanin was not synthesized. When melanin-containing conidia were used, the inhibitory effect of the cytochalasin on the infection was lower (approximately 50%). At least two mechanisms of infection of the host cell were observed: typical phagocytosis and another process in which the fungi played a more active role. Infection by F. pedrosoi was also observed in the non-professional phagocytic MDCK epithelial cell line. Two types of cytoplasmic vacuoles which contained parasites were seen in thin sections of host cells infected with F. pedrosoi: a ‘tight’ type and a ‘loose’ type. At least 200 conidia-containing vacuoles were analysed by transmission electron microscopy. The ‘tight’ type was observed in 75% of the vacuoles of non-treated macrophages, suggesting an association with classical phagocytosis. On the other hand, the ‘loose’ type vacuole was seen in 75% of the vacuoles present in cytochalasin treated macrophages and seemed to be related to induced phagocytosis or active penetration by the fungi.