rDNA-RFLP and ITSI sequencing of species of the genus Fonsecaea, agents of chromoblastomycosis

Abstract

Amplicons of SSU and ITS1 +2 rDNA of 13 strains of Fonsecaea pedrosoi and three strains of F. compacta were digested with seven restriction enzymes. In addition, the ITS1 region of 14 strains was sequenced. With both methods significant variation was found which, however, did not coincide with established species limits based on morphology.

Keywords:

• chromoblastomycosis
• Fonsecaea
• ITS1 sequencing
• PCR-RFLP
• taxonomy