Presence of the diatom genus Pseudo-nitzschia and particulate domoic acid in the Golden Horn Estuary (Sea of Marmara, Turkey)

Abstract

The potentially toxic diatom genus Pseudo-nitzschia and particulate domoic acid (pDA) concentrations were investigated weekly to bi-weekly from October 2013 to September 2014 in the Golden Horn Estuary. Pseudo-nitzschia species were detected in 324 of the 666 samples (∼49%). Two species, Pseudo-nitzschia calliantha and Pseudo-nitzschia pungens, were identified based on the scanning electron microscopy examination. A bloom of P. calliantha occurred between May and June, average cell density, 1.1 × 10⁶ cells L⁻¹ in May. Pseudo-nitzschia calliantha was commoner than P. pungens almost throughout the study period. They occurred over a wide temperature range (7.4–26.3°C) and moderate salinity (5.9–19.9). The highest cell density occurred at 14.7–19.6°C and 18.5–19.0 salinity, in the lower and middle estuary in winter and late spring. Pseudo-nitzschia species were found in very low densities in the upper estuary, which has variable salinity and low light availability due to high concentrations of suspended material. The pDA concentration in seawater was 0.03–1.0 µg L⁻¹. The distribution pattern of pDA coincided with the presence of Pseudo-nitzschia. Pseudo-nitzschia abundance was low in October, when pDA was highest. There was a highly positive correlation between Pseudo-nitzschia abundance and salinity/Secchi depth/pH (p ≤ .001), but a negative correlation with inorganic nutrients and Si:N (p < .001). pDA was positively correlated with temperature/salinity and Secchi depth, but negatively correlated with inorganic nutrients (p < .001). Low light availability and highly variable salinity may limit the growth of Pseudo-nitzschia in the upper estuary and the presence of pDA throughout the study area may be related to transport by particulate matter.

Keywords:

• diatom
• domoic acid
• Golden Horn Estuary
• Pseudo-nitzschia, Sea of Marmara

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK) [Project number: 113Y091] and Research Fund of Istanbul University [Project number: ACIP-41804].