Effect of growth hormone on brain oedema caused by a cryogenic brain injury model in rats

Abstract

Although growth hormone (GH) is a potential agent to counteract the catabolic response to general and head injuries, the complications of GH treatment have been little studied. To evaluate the safety of GH in head trauma patients, it was investigated whether GH affects brain oedema caused by brain injury, using a rat freeze-injury model. Male Wistar rats (n = 28) were divided into four groups according to the substance injected (GH vs. saline) and whether brain was injured or not. GH (0.8IU/kg) or saline was injected subcutaneously every 24 hours. In the injury groups, immediately before the first injection of GH, an aluminum rod cooled to-50 C was placed on the right parietal region for 4 minutes, under anaesthesia. At 96 hours after the insult, brain and skeletal muscle were excised and their water content was measured, by drying. Freeze injury of the brain caused an increase in water content in skeletal muscle. GH injection augmented this oedema in skeletal muscle. Freeze injury of the brain also caused an increase in water content in the injured and non-injured hemispheres of brain and cerebellum. GH administration did not exacerbate brain oedema caused by brain injury in this model.