ABSTRACT
Objective
This study investigated the effects of ghrelin on oxidative stress, working memory, inflammatory parameters, and neuron degeneration.
Methods
TBI was produced with the weight-drop technique. Rats in the G+TBI and TBI+G groups received ghrelin for 7 or 2 days, respectively. The control group received saline. On the 8th day of the study, the brain and blood tissue were taken under anesthesia.
Results
A significant increase in brain GSH-PX, MDA, IL-1β, TGF-β1, and IL-8 levels and a significant decrease in CAT levels were found in the TBI group compared to the control. Serum MDA, GSH, IL-1β, and IL-8 levels were increased with TBI. Ghrelin treatment after TBI significantly increased the serum GSH, CAT, GSH-PX, and brain GSH and CAT levels, while it significantly decreased the serum MDA, IL-1β, and brain MDA, TGF-β1, and IL-8 levels. Histological evaluations revealed that ghrelin treatment led to a reduction in inflammation, while also significantly ameliorating TBI-induced neuron damage and vascular injuries. Immunohistochemistry staining showed that GFAP staining intensity was significantly increased in the cortex and hippocampus in TBI, and GFAP immunoreactivity was decreased with ghrelin treatment.
Conclusion
The results from this study suggested that ghrelin may have curative effects on TBI.
Acknowledgments
This work has been supported by Van Yuzuncu Yil University Scientific Research Projects Coordination Unit under [grant number TSA-2021-9340]. The authors want to thank the Van Yuzuncu Yil University Scientific Research Projects Coordination Unit for the financial support.
The study procedures were approved by the Van Yuzuncu Yil University Ethical Committee [29/10/2020- 2020/10-10].
Disclosure statement
The authors report there are no competing interests to declare.
Correction Statement
This article has been republished with minor changes. These changes do not impact the academic content of the article.