Effects of a Simulated Tennis Match on Lymphocyte Subset Measurements

Abstract

Tennis is an activity requiring both endurance and anaerobic components, which could have immunosuppressive effects postexercise.

Purpose

The purpose of this investigation was to determine the effect of a simulated tennis match on apoptotic and migratory markers on lymphocyte subsets.

Method

Male high school (n = 5) and college (n = 3) tennis players (M _age = 18.9 ± 3.3 years) completed 10 sets of a tennis protocol including serves, forehand strokes, and backhand groundstrokes with 1-min rest periods between sets. Apoptosis antigen 1 receptor (CD95) and chemokine receptor fractalkine (CX₃CR1) expression was analyzed on helper T lymphocytes (CD4+), cytotoxic T lymphocytes (CD8+), and B lymphocytes (CD19+) twice, at resting baseline and immediately after all 10 sets of the tennis protocol.

Results

An increase was observed in each lymphocyte subtype (p <  .02, effect size = .41), and comparison of absolute changes revealed increases in CD4+/CD95+, CD8+/CD95+, and CD8+/CX₃CR1 lymphocytes following the tennis protocol (p <  .01, effect size = .43), but not in CD19+ cells.

Conclusions

A simulated tennis match has adequate intensity to induce immune modulations in terms of increased cell death and cellular migration in T lymphocyte subsets. Lymphocytopenia following tennis play is influenced by both apoptotic and migratory mechanisms.

Keywords:

• apoptosis
• cell migration
• lymphocytopenia