Choroidal Ganglion Cells in Prenatal, Young, and Middle-Aged Human Donor Eyes

Abstract

Purpose: In the current study, the appearance and development of choroidal ganglion cells (CGCs) was investigated in eyes of 18 human donors between the 13th week of gestation (wog) and 42 years of age. Methods: The number and diameter of CGCs was evaluated in scleral and choroidal whole mounts stained for NADPH diaphorase. To demonstrate the synaptic input of the CGCs, sections were stained with antibodies against synaptophysin, vesicular acetylcholin transporter, tyrosin hydroxylase, and vesicular monoaminergic transporter 2. Results: Clusters of small CGCs were first seen in the 18th wog next to the nasal and temporal long ciliary nerves. Immunohistochemistry in the 25th wog revealed 3298 and 5429 nitric oxide synthase/NADPH diaphorase positive CGCs, surrounded by numerous cholinergic and aminergic boutons. The number of CGCs decreased to 1000–2500 after birth. During postnatal development, the CGCs spread into small groups, distributed all over the choroid. The size of CGCs increased markedly up to adulthood. Conclusions: CGCs appear late during choroidal development, in parallel with the differentiation of the outer vascular layers. This coincidence might point to the primary role of the CGCs as specific vasoregulators in species with a well-developed fovea centralis.

KEYWORDS:

• choroid
• development
• innervation