Reliability of Different Image Analysis Methods for Scanning Laser Doppler Flowmetry

Andreas J. Kreis Centre for Eye Research Australia, University of Melbourne, Australia

Thanh Nguyen Centre for Eye Research Australia, University of Melbourne, Australia

Sophie Rogers Centre for Eye Research Australia, University of Melbourne, Australia

Jie Jin Wang Centre for Eye Research Australia, University of Melbourne, Australia; Centre for Vision Research, Westmead Millennium Institute, University of Sydney, Australia

Joanna Harazny University Eye Hospital of Erlangen, University of Erlangen, Germany

Georg Michelson University Eye Hospital of Erlangen, University of Erlangen, Germany

H. M. Omar Farouque Department of Cardiology, Austin Health Hospital, University of Melbourne, Australia

Tien Y. Wong Centre for Eye Research Australia, University of Melbourne, Australia; Singapore Eye Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

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Abstract

Purpose: To assess reliability and reproducibility of different analysis methods for retinal capillary flow, volume, and velocity from scanning laser Doppler flowmetry (SLDF) topography. Material and Methods: SLDF topography analysis using the default retinal flowmeter (HRF) were compared to that using automatic full-field perfusion image analyzer (AFFPIA) and quantified as intraclass correlation coefficients (ICC). Results: The AFFPIA full-field method had the highest reliability, with ICC 0.99 for capillary flow. The reproducibility using the AFFPIA full-field method was high ICC 0.74 for capillary flow. Conclusions: The AFFPIA full-field method is highly reliable and superior to the default HRF software.

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Reliability of Different Image Analysis Methods for Scanning Laser Doppler Flowmetry

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Reliability of Different Image Analysis Methods for Scanning Laser Doppler Flowmetry

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