Dexamethasone Increases Pigment Epithelium-Derived Factor in Perfused Human Eyes

Elizabeth M. Perruccio Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA

Laura Leigh S. Rowlette Duke University Medical Center, Durham, North Carolina, USA

Núria Comes Department of Ophthalmology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

Silvia Locatelli-Hoops Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA

Luigi Notari Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA

S. Patricia Becerra Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA

Teresa Borrás Department of Ophthalmology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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Abstract

Purpose: To investigate the effects of dexamethasone (DEX) on pigment epithelium-derived factor (PEDF) cDNA and secreted protein in human trabecular meshwork (TM). Methods: Anterior segment organ cultures were perfused with 0.1 μ M DEX (OD) and vehicle (OS). Primary human TM cells (HTM) were treated with DEX under similar conditions. PEDF mRNA and secreted PEDF protein were quantitated by RT-PCR and Western blot. Results: PEDF mRNA and secreted PEDF protein levels were significantly higher in DEX over vehicle-treated cultures. In contrast, DEX decreased the activity of a 92-kDa gelatinolytic zymogen in organ culture effluents. Conclusion: DEX action in the human TM might include a PEDF-mediating pathway.

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