In Vivo Confocal Microscopic Grading System for Standardized Corneal Evaluation: Application to Toxic-Induced Damage in Rat

Aude Pauly Paris-Descartes University, UMRS 872, Paris, France; Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University of Paris, Paris, France

Antoine Labbe Paris-Descartes University, UMRS 872, Paris, France; Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University of Paris, Paris, France; Quinze-Vingts National Ophthalmology Hospital, University of Paris, Paris, France

Christophe Baudouin Paris-Descartes University, UMRS 872, Paris, France; Quinze-Vingts National Ophthalmology Hospital, University of Paris, Paris, France; Ambroise Paré Hospital, APHP, Ouest School of Medicine, University of Versailles, Paris, France

Hong Liang Paris-Descartes University, UMRS 872, Paris, France; Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University of Paris, Paris, France; Quinze-Vingts National Ophthalmology Hospital, University of Paris, Paris, France

Jean-Michel Warnet Paris-Descartes University, UMRS 872, Paris, France; Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University of Paris, Paris, France; Quinze-Vingts National Ophthalmology Hospital, University of Paris, Paris, France

Françoise Brignole-Baudouin Paris-Descartes University, UMRS 872, Paris, France; Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University of Paris, Paris, France

Abstract

Purpose: To propose an in vivo confocal microscopic scoring system for evaluation of irritant-induced corneal changes. Materials and Methods: Rat corneas were instilled with 0.01–0.5% benzalkonium chloride (BAC) and examined using high-resolution in vivo confocal microscopy (HRT-II) to measure corneal thickness and characterize corneal damage patterns. Severity scores were given for each predefined evaluation parameter and then totaled. Results: The scoring system revealed a dose-dependent effect of BAC and discriminated between high- and low-dose treatments. Conclusions: This HRT-II scoring standardizes damage evaluation at the cellular level, even when assessing irritating compounds at low concentrations.

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In Vivo Confocal Microscopic Grading System for Standardized Corneal Evaluation: Application to Toxic-Induced Damage in Rat

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In Vivo Confocal Microscopic Grading System for Standardized Corneal Evaluation: Application to Toxic-Induced Damage in Rat

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