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Current Eye Research Volume 34, 2009 - Issue 1
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Research Article

C5a, But Not C3a, Increases VEGF Secretion in ARPE-19 Human Retinal Pigment Epithelial Cells

Daniel N. Cortright Neurogen Corporation, Branford, Connecticut, USA
,
Robin Meade Neurogen Corporation, Branford, Connecticut, USA
,
Stephen M. Waters Neurogen Corporation, Branford, Connecticut, USA
,
Bertrand L. Chenard Neurogen Corporation, Branford, Connecticut, USA
&
James E. Krause Neurogen Corporation, Branford, Connecticut, USA
Pages 57-61 | Received 18 Jun 2008, Accepted 09 Oct 2008, Published online: 02 Jul 2009
 
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Abstract

Purpose: We examined the potential for the pro-inflammatory complement proteins C5a and C3a to increase VEGF expression in ARPE-19 cells. Materials and Methods: Expression of complement receptors in ARPE-19 cells was evaluated by RT-PCR. VEGF secretion from ARPE-19 cells treated with C5a or C3a was determined by ELISA. Results: C5a and C3a receptor, but not C5L2, were detected in human eye tissue and ARPE-19 cells. C5a, but not C3a, treatment increased VEGF secretion from ARPE-19 cells, an effect inhibited by the C5aR antagonist, NDT 9513727. Conclusions: C5a receptor mediates increased VEGF secretion from ARPE-19 cells, suggesting a role for the C5a receptor in the pathogenesis of macular degeneration.

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