Retinal Oxygen Saturation in Patients with Primary Open-angle Glaucoma Using a Non-flash Hypespectral Camera

ABSTRACT

Purpose/Aim: To investigate retinal vessel blood oxygen saturation in patients with primary open-angle glaucoma (POAG) and healthy controls.Materials and Methods: A novel non-flash hyperspectral retinal camera was used to image 17 healthy individuals (mean age 69.3 ± 6.1 years) and 22 patients with stable POAG (mean age 69.2 ± 5.8 years) at 548, 569, 586, 600, 605, and 610 nm wavelengths. POAG patients were grouped as mild–moderate (n = 13) and moderate–severe (n = 9) based on Humphrey 24-2 visual field results (mean deviation [MD] < −6 and MD ≥ −6; respectively). Optical density values were extracted using Image-J software for blood oxygen saturation (SO₂) determination. Arteriolar and venular SO₂ were measured within 1.5 optic nerve head diameters from the disc margin along the vessels in the inferior temporal quadrant of the tested eye per subject. Analysis of variance (ANOVA), student t-test, and Pearson’s correlation were used for statistical analysis of the data (p < 0.05).

Results: Venular, arteriolar, and arteriovenous (AV) differences in SO₂ measurements were not significantly different between controls and the combined POAG groups (p > 0.06 for all). However, mean venular SO₂ was significantly higher in the POAG with MD ≥ −6 dB when compared to controls and patients with mild glaucoma (p = 0.005). The AV differences were significantly lower in patients with more severe field defect (p = 0.006) compared to the remaining groups. No differences were found in the mean arteriolar SO₂ between the groups (p = 0.155). Significant correlations were found only between higher visual field MD values and higher venular SO₂ (p = 0.048) but not the remaining SO₂ measurements.

Conclusion: Patients with POAG and moderate–severe visual field defect had higher venular SO₂ compared to those with mild–moderate defect and controls. This would indicate reduced oxygen consumption in more advanced glaucoma likely as a result of ganglion cell degeneration.

KEYWORDS:

• Hyperspectral retinal camera
• primary open-angle glaucoma
• retinal oxygen saturation

Acknowledgment

The authors would like to thank Jean-Phillip Sylvestre for contribution to preparation of this manuscript.

Declaration of interest

Ayda M Shahidi: None.Chris Hudson: Consultant and Scientific Advisor for Optina Diagnostics.Faryan Tayyari: None.John G. Flanagan: None.