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Extra-Ocular Structures

Bupivacaine Injection in the Extra Ocular Muscle of Rabbits: Analysis of Global and Orbital Layers

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Pages 950-954 | Received 18 Aug 2019, Accepted 23 Nov 2019, Published online: 10 Dec 2019
 

ABSTRACT

Purpose

To verify the cross sectional area (CSA) of fibers positive for myosin type 1 in the global and orbital layers after bupivacaine (BUP) injection in the superior rectus of the right eye of rabbits.

Materials and Methods

A total of 40 rabbits were studied; 32 specimens received a 0.3 mL injection of BUP at 1.5% in the superior rectus of the right eye and were euthanised after 7, 28, 60 and 92 days. Another eight rabbits were used as the control group. Immunohistochemical analysis was performed using anti-slow myosin (type 1) antibodies. The ImageJ software was used to measure the CSA of the fibers. Six slides were excluded from the analysis. Finally, 7,204 fibers were counted and statistically analysed.

Results

The mean CSA was 0.040 µm2 for all the studied fibers. For the orbital layer, mean CSA was 0.038 µm2, whereas the global area measured 0.042 µm2. No statistically significant differences were observed when comparing the orbital layer groups. However, when comparing the mean CSA in the global layer of the control group with the 60-day group an increase was found (0.0339 ± 0.0062 versus 0.0503 ± 0.0114; p = .009).

Conclusion

This study revealed that 60 days after BUP injection, the extra ocular muscle of rabbits presented an increase in the CSA of myosin 1 type fibers in the global layer.

Declaration of interest

Luisa Moreira Hopker: None

Marco Aurélio Senff de Moraes: None

Rodrigo Nitsch: None

Gabriela Wahab Pasqual: None

Pamela Cavagnari: None

Solena Ziemer Kusma: None

Luciane Moreira: None

Edmar Zanoteli: None

Norma Allemann: None

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

CnPQ - Brazilian National Council for Scientific and Technological Development.

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