The Biochemical Characteristics in Experimental Keratomycosis

Abstract

Purpose

To investigate the biochemical characteristics in experimental keratomycosis.

Methods

Experimental mice were injected with Fusarium solanum solution Controls mice received liposomes containing phosphate-buffered saline (PBS-LIP). Raman spectroscopy was used to analyze the biochemical characteristics. The infiltration of inflammatory cells was analyzed by histopathology. Cytokine mRNA levels were detected by real-time polymerase chain reaction.

Results

Raman Spectroscopy: In the experimental group, collagen, lipids, amide I and III were decreased, amide II, hyper proline amino acids, and arginine were increased, and proline and phenylalanine were significantly increased on day 3. Histopathology: more severe keratomycosis developed in the experimental group than in the control group. Statistically significant mRNA expression of Collagen4\MMP2\MMP9\TIMP1.MMP9 was negatively correlated with the secretion of Collagen4.

Conclusions

Matrix metalloproteinases are involved in biochemical changes in keratomycosis.

Keywords:

• Fungal keratitis
• matrix metalloproteinases
• Raman spectroscopy

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Additional information

Funding

This work was supported by School of Medical Technology and Engineering Foundation of Fujian Medical University, Fuzhou, China [grant nos: 2018xy002].