A new thin sectioning method for observation of higher resolution images in bone histomorphology

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DISCLOSURE STATEMENT

No potential conflict of interest was reported by the author(s).

ACKNOWLEDGMENTS

We are grateful to S. Fujiwara (Nagoya University Museum, Aichi, Japan) for supervising the first author. S. Fujiwara, J. Hatase (Hiroshima City Asa Zoological Park, Hiroshima, Japan), K. Sone (Nagoya Biodiversity Center, Aichi, Japan), and Y. Yokohata (University of Toyama, Toyama, Japan) provided us the bone materials. K. Nakamura (Hokkaido University, Hokkaido, Japan) and Y. Koketsu (Nagoya University, Aichi, Japan) advised the method to make thin sections in paleontological and petrological studies. Y. Koketsu and D. Mills (Queen Mary University of London, London, UK) supported the equipment used in this study. We are grateful to A. Huttenlocker (University of Southern California, California, U.S.A.), H. N. Woodward (Oklahoma State University Center for Health Sciences, Oklahoma, U.S.A.), M. Whitney (Harvard Museum of Comparative Zoology, Harvard University, Massachusetts, U.S.A.), and an anonymous reviewer for the critical and beneficial comments as editors and reviewers. Japan Society for the Promotion of Science (JSPS) fellowship (grant no. 21J14927) supported this research.

AUTHOR CONTRIBUTIONS

DN launched the research project, prepared the specimens and chemicals used in this study, and thin sections, created figures, interpretation, and drafted the manuscript. AB raised and discussed the issues of conventional thin sectioning preparation, designed the solution, prepared the specimen, chemicals used in this study, and instruments for thin sectioning, and composed parts of the manuscript. Both authors edited the manuscript.

SUPPLEMENTARY FILES

Supplementary_File_1.docx: Figures S1 and S2

DATA AVAILABILITY STATEMENT

The high-resolution images of thin sections used in this study are available on Morphobank: http://morphobank.org/permalink/?P4522.