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Abstract
Humic substances (HS) as a background electrolyte in capillary electrophoresis afford the possibility to characterise HS/reagent interactions. Thus, added Pb(NO3)2 reveals specific characteristic regions in electropherograms that correlate with the salt concentration. These regions form because of the on-capillary complexation of Pb2+ with HS: fulvic acid, hymatomelanic acid and humic acid. The formation of two complexes (Pb/HS fractions I and II), which have different mobility, was observed. Each of the HS fractions contains those complexes in a different ratio. Differences in the electropherograms apparently reflect structural differences in the fractions. The peak areas of both complexes have a good linear correlation with the concentration of injected Pb2+.
Acknowledgements
We thank the Estonian Ministry of Education and Research for financial support (Doctorate Grant No. 0172062s01) and Kati Helmja for technical assistance.