Bioaccumulation and adverse effects of trace metals and polycyclic aromatic hydrocarbons in the common onion Allium cepa as a model in ecotoxicological bioassays

Abstract

The common onion Allium cepa can be easily used in ecotoxicological bioassays to evaluate the bioavailability and adverse effects of pollutants and complex mixtures like waste, industrial effluent or coal-mining drainage that contain elevated levels of trace metals and polycyclic aromatic hydrocarbons (PAHs). In this study, onions were exposed for 7 days to individual metals (1 µg⋅mL⁻¹), i.e., aluminium, copper, iron and manganese, or PAHs (1.5 ng⋅mL⁻¹), i.e., benzo[a]antracene and benzo[a]pirene. Biological effects, measured as growth inhibition of roots and leaves, were integrated with analysis of bioaccumulation in roots, bulbs and leaves. Copper, iron and benzo[a]pirene caused a significant inhibition in root development of newly formed tissues, whereas only slight variations were caused by other chemicals; the number of new root filaments and the length of the leaves did not show significant variations, thus not representing sensitive parameters to evaluate adverse effects of pollutants in A. cepa. Chemicals bioaccumulation was always significant in roots, whereas levels in bulbs and leaves exhibited increased levels only for manganese, and a decrease for aluminium. The overall results confirmed the sensitivity of the bioassays with A. cepa, suggesting their utility for future applications to evaluate the adverse effects of complex mixtures containing metals or PAHs.

Keywords:

• Allium cepa
• trace metals
• polycyclic aromatic hydrocarbons
• bioaccumulation
• toxicity
• bioassay

Acknowledgements

The authors wish to thank Prof. Francesco Regoli (Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, Ancona, Italy) for his support and the critical comments. This work was partially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil), project number140204/2005-9.