Effect of stearoyl-coenzyme a desaturase 1 (SCD1) on the function of mast cells

Abstract

Background: The prevalence of childhood asthma and obesity is increasing, while obesity increases the risk and severity of asthma. Lipid metabolism has been considered as an important factor in the pathogenesis of obesity-associated asthma. Stearoyl-CoA desaturase 1 (SCD1) is a rate-limiting enzyme that catalyzes the production of monounsaturated fatty acids (MUFA).Methods: In the present study, the microarray data retrieved from the Gene Expression Comprehensive Database (GEO) was analyzed to further clarify the impact of SCD1 on Mast cell activation related lipid mediators and the correlation between SCD1 and obesity asthma in the population.Results: SCD1 was highly expressed in IgE-activated bone marrow-derived mast cells (BMMCs). Meanwhile, SCD1 was also verified expressed highly in dinitrophenyl human serum albumin (DNP-HAS) stimulated RBL-2H3 cells. The expression of SCD1 was up-regulated in peripheral blood leukocytes of asthmatic children, and was positively correlated with skinfold thickness of upper arm, abdominal skinfold and body mass index (BMI). Inhibition of SCD1 expression significantly suppressed the degranulation, lipid mediator production, as well as the migration ability in DNP-HAS-stimulated RBL-2H3 cells.Conclusion: SCD1 is involved in obese-related asthma through regulating mast cells.

Keywords:

• Stearoyl CoA desaturase 1 (SCD1)
• obesity
• asthma
• mast cells
• lipid mediators

Author contributions

X.Z. and A.L. performed experiments, statistical analysis, made the figures and tables, and drafted the manuscript. Y.Z. collected clinical data and revised the manuscript. F.L. and D.Z. participated in the study design and determined the clinical status for each child in the study. C.X. contributed to the study design and interpretation of the data. H.T. designed the study, analyzed the data, and revised the manuscript. All authors read and approved the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

All data, models, and code generated or used during the study appear in the submitted article.

Additional information

Funding

This study was supported by the Research Project of Jiangsu Maternal and Child Health Association (FYX201905).