Royal jelly inhibited N-acetylation and metabolism of 2-aminofluorene in human liver tumor cells (J5)

Abstract

The present study was carried out to evaluate the question of whether or not royal jelly affects N-acetylation and metabolism of 2-aminofluorene (2-AF) in the human liver tumor cell line (J 5). N-acetylation and metabolism of 2-AF in intact J5 cells was determined by using high performance liquid chromatography for the amounts of acetylated and nonacetylated 2-AF and profile of 2-AF metabolism. The results indicated that royal jelly displayed a dose-dependent inhibition of N-acetylation of 2-AF in J5 cells. Royal jelly also decreased the profile of 2-AF metabolites in J5 cells. This report is the first demonstration which showed that royal jelly affects N-acetylation of 2-AF in human liver tumor cells (J5).

Keywords:

• N-acetyltransferase
• royal jelly
• liver tumor cells
• arylamine (N-acetyl-2-aminofluorene and 2-aminofluorene)