Effects of nonylphenol on the brain cytochrome P450 gene expression in F1 generation rats

Abstract

In order to explore the effects of nonylphenol (NP) on brain cytochrome P450 gene expression in F1 generation rats microarray analysis techniques were used. mRNA were extracted from the brain of 2-day-old F1 generation rats whose F0 male generation were treated with NP, then reversely transcribed to cDNA and labeled with cy5 and cy3 for fluorescence. Subsequently, the cDNA probes were hybridized to the mouse 40S cDNA microarray; and fluorescent signals produced by cy5 and cy3 were scanned and analyzed. Sixteen identified genes were found to be expressed differently from control, including three cytochrome P450 genes, in which two were up-regulated and one down-regulated. Data suggest that NP affects the expression of some cytochrome P450 genes in rat brain when administered perinatally.

Keywords:

• Nonylphenol
• gene expression
• cytochrome P450