Therapeutic effect of melatonin against aluminum-induced neurotoxicity in cerebellum of albino mice

Abstract

The therapeutic effect of melatonin (MEL) against aluminum (Al)-induced neurotoxicity was investigated in mouse cerebellum. Two groups of male albino mice were intraperitoneally injected with Al acetate or MEL alone, at doses of 3.5 or 7 mg kg⁻¹ day⁻¹, respectively, for 6 weeks. During this period, another group of animals received a combination of both Al and MEL (3.5 + 7 mg kg⁻¹ day⁻¹). At the end of the treatment cerebellum was removed and processed to examine the oxidative stress markers: superoxide dismutase (SOD), catalase (CAT), and thiobarbituric acid-reactive substances (TBA-RS). Oxidative stress increased significantly with administration of Al which was estimated by increased TBA-RS and reduction in the activities of SOD and CAT. However, these alterations were significantly reversed significantly following MEL treatment which was observed in co-administered group. Protective effects of MEL were also observed at electron microscopic level. Ultrastructural studies revealed an increase in vacuolization, chromatin condensation within the nucleus, degenerated purkinje cell, degenerated axon and degenerated granule cells in the cerebellum of Al-treated mice group whereas concurrent administration of MEL with Al reduced these changes. The results of the present investigation emphasize the potential use of MEL as a supplement in therapy of free radical based neurological disorders in which oxidative stress is involved.

Keywords:

• aluminum
• oxidative stress
• melatonin
• cerebellum

Acknowledgements

S. Priyanka is highly thankful to University Grants Commission (UGC) Rajiv Gandhi National Fellowship (India) for providing a Junior Research Fellowship (JRF) and supporting this research work.