Tissue-specific stress and hepatic DNA damage in Pelteobagrus fulvidraco caused by low concentrations of cadmium

Abstract

Physiological stress and DNA damage in Pelteobagrus fulvidraco induced by continuous exposure to cadmium at concentrations of 170 and 1700 µg/L for up to 28 days was evaluated. The activities of acetylcholinesterase and monoamine oxidase in brain tissue, Na⁺-K⁺-ATPase and glutathione in gill tissue, and superoxide dismutase and catalase in liver tissue were measured. In studying random amplified polymorphic DNA to evaluate cadmium-induced hepatic genotoxicity, both the appearance of new bands and the disappearance of existing bands were observed, as well as increased levels of monoamine oxidase and Na⁺-K⁺-ATPase and decreased activities of antioxidant enzymes. The results suggest that continuous exposure to cadmium at the studied levels can induce biochemical and physiological changes and DNA damage in P. fulvidraco.

Keywords:

• cadmium
• fish
• physiological stress
• genotoxicity
• RAPD technique

Acknowledgments

This study was supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201503108); the China Three Gorges Project Corporation (No.07011034); and the Ministry of Education, Youth and Sports of the Czech Republic-projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024) and “CENAKVA II” (No. LO1205 under the NPU I program).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Special Fund for Agro-scientific Research in the Public Interest [grant number 201503108]; China Three Gorges Project Corporation [grant number 07011034]; Ministry of Education, Youth and Sports of the Czech Republic-projects “CENAKVA” [grant number CZ.1.05/2.1.00/01.0024], “CENAKVA II” [grant number LO1205 under the NPU I program].