Role of the necrosis factor-κB pathway in dibutyl phthalate mediated effects on human glioma cells

Abstract

The human glioma cell line U251 was incubated for 12 h and 24 h in medium containing dibutyl phthalate at concentrations of 25 µmol/L and 100 µmol/L. Decreased cell viability and increased oxidative stress show the cytotoxicity of dibutyl phthalate. Nuclear factor-κB and nuclear factor-κB pathway-related proteins were altered, which could be attenuated by vitamin E at 20 µmol/L. Thus, dibutyl phthalate induces cytotoxicity through activation of nuclear factor-κB and vitamin E exerts neuroprotection against dibutyl phthalate-mediated cytotoxicity in vitro.

Graphical Abstract

Keywords:

• Dibutyl phthalate
• U251 cells
• nuclear factor-κB
• oxidative stress
• vitamin E

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Health and Family Planning Commission Scientific Research Project of Hubei Province (WJ2017Z027), the College Outstanding Young and Middle-aged Innovative Research Team Project of Hubei Province (T201717), and the Science and Technology Research Project of Education Department of Hubei Province (B2018172).