Diflubenzuron and the biological activity of Azospirillum brasilense

C.E. Sanchez Group of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain

B. Rodelas Group of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain

M.V. Martinez‐Toledo Group of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain

V. Salmeron Group of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain

J. Gonzalez‐Lopez Group of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain

The effect of 10, 50, 100 and 200 μg diflubenzuron/ml on growth, acetylene‐reduction activity (ARA) and ATP content was investigated with N‐free and NH₄ ⁺‐batch cultures of Azospirillum brasilense. Difiubenzur‐on at concentrations of 10 to 200 μg/ml significantly increased growth, ARA and amounts of ATP in cells grown for 24 and 36 h. However, these effects were not significant in cells grown for 48 and 72 h. Our data show that A. brasilense can tolerate high concentrations of that insecticide.

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Diflubenzuron and the biological activity of Azospirillum brasilense

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