Foliar δ¹⁵N variations with stand ages in temperate secondary forest ecosystems, Northeast China

Abstract

Natural abundance of ¹⁵N changes strongly with gradients in soil or environmental conditions across large spatial and temporal scales, but variation in δ¹⁵N with stand age at intermediate scales is poorly understood. We analyzed soil and foliar δ¹⁵N along a secondary forest chronosequence in Northeast China in mid-growing seasons 2008 and 2009, to address two questions: how does foliar δ¹⁵N vary with stand age; and are the variations driven by compositional differences in species among stands, or by consistent changes in δ¹⁵N with plant growth forms, and within species. The results showed that community-level foliar δ¹⁵N depleted as stand age increased, and these responses were remarkably consistent within three contrasting plant growth forms (herbs, shrubs, and trees), and within individual species. In spite of the three plant growth forms sharing similar responses to the stand age, tree species showed smaller variations in foliar δ¹⁵N along stand ages than herbs and shrubs. Soil δ¹⁵N also significantly depleted with increasing stand age, which may drive the variations of foliar δ¹⁵N. In addition, mycorrhizal fungi discrimination against ¹⁵N may also partly affect the patterns of foliar δ¹⁵N along stand ages. The results clearly indicate that differences in foliar δ¹⁵N among different stand ages are dominantly driven by the δ¹⁵N variations at the species levels, which reflects the variations of soil δ¹⁵N and mycorrhizal association intensity and association type, not by compositional difference in species among stands.

Keywords:

• δ¹⁵N
• stand age
• plant growth form
• species composition
• ecosystem openness

Acknowledgements

This work was sponsored by the National Natural Science Foundation of China (30830085, 40901283). The authors thank all members in Research Group of Ecology & Management for Secondary Forests for their help in the fieldwork and excellent suggestions. They also thank Dr Edith Bai for her assistance with English language and academic suggestion to this manuscript.