Abstract
We examined the effectiveness of hepatocyte growth factor (HGF) in blocking production of transforming growth factor (TGF)-β1-induced collagen I, fibronectin, and α-smooth muscle actin (α-SMA) in the flexor tendon sheath of rabbits in vitro. Fibroblasts were obtained from the sheaths. Cell culture was supplemented with TGF-β1 5 ng/ml and increasing doses of HGF (10-40 ng/ml). The production of collagen I and fibronectin in supernatants culture were examined using an enzyme-linked immunosorbent assay (ELISA). α-SMA expression was assessed by western blot. TGF-β1 stimulated production of collagen I, fibronectin, and α-SMA greatly, while HGF significantly (p<0.05) reduced production of all components induced by TGF-β1 in a dose-dependent manner. This suggests that HGF effectively antagonises the action of TGF-β1 in cultured fibroblasts from flexor tendon sheaths. The results provide a cellular and molecular basis for HGF acting as a therapeutic agent for adhesions in flexor tendons.