Fibrin Glue (Tisseel^R) Added with Sodium Hyaluronate in Microvascular Anastomosing

Abstract

To test the effect on the handling properties of a two component fibrin glue, the viscosity was increased with sodium hyaluronate and the glue was applied to a microvascular anastomosis in rats. The femoral artery of each rat was anastomosed with three conventional sutures and then sealed with fibrin glue. Three glues with different viscosities were tested: original Tisseel^R fibrin glue (Immuno AG. Vienna); Tisseel^R with 0.9% sodium chloride added to the fibrinogen component; and Tisseel^R with a high molecular weight sodium hyaluronate (10 mg/ml, Healon^R, Pharmacia, Sweden) added to the fibrinogen component. The increased viscosity of the fibrin glue to which hyaluronate had been added resulted in a significantly higher patency rate 20 minutes after completion of the anastomosis (p<0.01), and reduced the amount of fibrin that entered the vessels.

Key words:

• fibrin glue
• hyaluronic acid
• viscosity
• microvascular
• anastomosis