A Method for Determining Plasma Proteins in Inflammatory Exudate Absorbed by Sephadex

Karin Ganrot Department of Clinical Chemistry, University of Lund, Malmo General Hospital, Malmo, Sweden; Department of Plastic Surgery, University of Lund, Malmo General Hospital, Malmo, Sweden

Sten Jacobsson Department of Clinical Chemistry, University of Lund, Malmo General Hospital, Malmo, Sweden; Department of Plastic Surgery, University of Lund, Malmo General Hospital, Malmo, Sweden

Ulf Rothman Department of Clinical Chemistry, University of Lund, Malmo General Hospital, Malmo, Sweden; Department of Plastic Surgery, University of Lund, Malmo General Hospital, Malmo, Sweden

Abstract

Dry cross-linked dextran (Sephadex pharmaceutical grade, Debrisan®) was applied to weeping sores. After some exudate had been absorbed the gels were removed. The amount of exudate was determined by isotope dilution techniques. The accuracy of the method was tested by determination of plasma proteins in samples of diluted serum containing known concentrations of some plasma proteins. The method seems suitable for determining various high and low molecular weight constituents of exudates.

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A Method for Determining Plasma Proteins in Inflammatory Exudate Absorbed by Sephadex

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A Method for Determining Plasma Proteins in Inflammatory Exudate Absorbed by Sephadex

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