Sec-Page Characterization of Lake Aquatic Humic Matter Isolated with Xad-Resin and Tangential Membrane Ultrafiltration

Juhani Peuravuori Department of Chemistry, Laboratory of Physical Chemistry, University of Turku, FIN-20014, Turku, Finland

Kalevi Pihlaja Department of Chemistry, Laboratory of Physical Chemistry, University of Turku, FIN-20014, Turku, Finland

Olga Trubetskaya Branch of the Institute of the Bioorganic Chemistry, Russian Academy of Sciences, 142292, Pushchino, Moscow region, Russia

Olga Reznikova Branch of the Institute of the Bioorganic Chemistry, Russian Academy of Sciences, 142292, Pushchino, Moscow region, Russia

Gaida Afanas'eva Branch of the Institute of the Bioorganic Chemistry, Russian Academy of Sciences, 142292, Pushchino, Moscow region, Russia

Oleg Trubetskoj Institute of Basic Biological Problems, Russian Academy of Sciences, 142292, Pushchino, Moscow region, Russia

Abstract

Several aquatic heterogeneous humic-solute fractions with known molecular-size ranges were fractionated further by coupled size-exclusion chromatography (SEC) and polyacrylamide gel electrophoresis (PAGE). The results of SEC-PAGE, obtained for several humic-solute fractions, corresponded reasonably well to the known molecular-size distributions of the dissolved organic matter (DOM). Despite the limited resolving power PAGE appears to offer an alternative coarse method, beside SEC, for fractionation of the heterogeneous humic matter (HM).

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Sec-Page Characterization of Lake Aquatic Humic Matter Isolated with Xad-Resin and Tangential Membrane Ultrafiltration

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Sec-Page Characterization of Lake Aquatic Humic Matter Isolated with Xad-Resin and Tangential Membrane Ultrafiltration

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