81
Views
15
CrossRef citations to date
0
Altmetric
Original Articles

Characterization of graphite electrodes modified with laccases from Trametes hirsuta and Cerrena unicolor and their use for flow injection amperometric determination of some phenolic compounds

, , , &
Pages 753-770 | Received 14 Sep 2004, Accepted 10 Dec 2004, Published online: 25 Jan 2007
 

Abstract

Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 μL of solutions with different phenolic substrates into the carrier citrate buffer solution with a working potential of −50 mV vs. Ag|AgCl. Statistical aspects were applied and the Michaelis–Menten constants evaluated were correlated to the chemical structure of the investigated phenolic compounds.

Acknowledgements

The authors thank the Swedish Research Council (VR), the Swedish International Development Cooperation Agency (SIDA, 348-2003-4947) and the European Community (ICA2-CT-2000-10050) for financial support. The Swedish Institute (SI) is acknowledged for the support of a postdoctoral fellowship for B.H.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.