Complexation of gadolinium(III) ions on top of nanometre-sized magnetoliposomes

Abstract

The complex of diethylenetriaminepentaacetate (DTPA) with the paramagnetic gadolinium ion [Gd(III)] is a well-known blood pool contrast agent for magnetic resonance imaging (MRI). To obtain MRI pictures from other anatomical structures, for instance from tissues containing cells with phagocytic activity, larger colloidal complexes have to be constructed. Therefore, in view of modifying the physiological behaviour, the DTPA chelate was first hydrophobized by covalently linking it to phosphatidylethanolamine (PE), and the resulting conjugate was then incorporated into nanometre-sized, sonicated phospholipid vesicles. Qualitative information on the affinity of the PE–DTPA derivative for Gd(III) ions was derived from competition experiments using the dye Arsenazo. Furthermore, it was found that only the membranotropic adducts residing in the outer shell of the vesicle bilayer are accessible to the lanthanide ion. The vesicular particulate was also used as a vehicle to transport PE–DTPA into the coating of so-called magnetoliposomes which consist of nanometre-sized iron oxide cores onto which a phospholipid bilayer is strongly chemisorbed. After loading the resulting structures with Gd(III), this new type of magnetoliposome may offer unique potentialities as a novel bi-label MRI contrast medium.

Keywords:

• Diethylenetriaminepentaacetate (DTPA)
• Gadolinium
• Liposome
• Magnetoliposome
• MRI contrast agent
• Phospholipid vesicle

Acknowledgement

Supported by SBO project no. IWT/030238 to MDC. SJHS is the recipient of a research grant from the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaandenen).