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Abstract
The white rot fungus Phanerochaete chrysosporium is able to degrade PCBs in water and in soil. This study aims at estimating the fractioning of truly degraded PCBs, adsorbed PCBs and residual PCBs in an aqueous bioremediation assay with Phanerochaete chrysosporium. Di-, tri- tetra-, penta-, hexa-, hepta-chlorinated biphenyls (IUPAC numbers: PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, PCB180) were extracted from 500 mL aqueous bioremediation assays using a liquid–liquid extraction with n-hexane. Analyses were performed by gas chromatography coupled to mass spectrometry. The study reveals that the adsorbed PCBs fraction ranges from 42% to 54%, whereas the degraded one ranges from 39% to 49%. No PCBs were detected in the residual water (limit of detection: 13 ng L−1).
Acknowledgements
This work is supported by Agence National de la Recherche Technologique with Grant No. 46/2007 and by OSEO with Grant No. A0703011. We would like to thank Ecole National de Formation Agronomique for their technical support.
