Abstract
exhibited catalytic effect on the cracking reaction of double-stranded DNA (dsDNA) to form a short single-stranded DNA (ssDNA) that protected the nanogold (NG) to produce stable NGssDNA conjugate. The un-protected NG was aggregated to form NG aggregates (NGA) that appeared a resonance scattering (RS) peak at 542 nm. Unlike NGA, the NGssDNA exhibited a strong catalytic activity on the Cu2O particle reaction of Fehling reagent-glucose that can be monitored by RS technique at 608 nm. When the
concentration increased, the NGssDNA increased, and the RS intensity at 608 nm increased. The increased RS intensity ΔI
608 nm was linear to the
concentration in the range of 15–200 pmol L−1, with a regression equation of ΔI
608 nm = 1.24 C + 4.6, and a detection limit of 5 pmol L−1. This new RS assay was applied to assay
in water sample, with satisfactory results.
Acknowledgements
This work supported by the National Natural Science Foundation of China (Nos. 21075023, 20965002, 20865002), Natural Science Foundation of Guangxi (No.0991021z), the Research Funds of Guangxi Water Conservancy and the Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education (No. 1001Z019).