Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

Xilin XiaoCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaCorrespondence[email protected] [email protected]
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Jinhua XueCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

Dexin DingCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaCorrespondence[email protected] [email protected]
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Lifu LiaoCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

Xialing MengCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

Liangzhuan LiuCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

Bin ZhouCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

Bo HeCollege of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang City, 421001, P. R. ChinaView further author information

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Abstract

The interactions of metallothioneins with Eosin Y were studied by the absorption spectra and resonance light scattering (RLS) spectra methods. A direct RLS spectra method was applied for the determination of trace metallothioneins. The interaction of Eosin Y and metallothioneins enhanced the RLS intensity of system in Britton-Robinson buffer (pH 3.91), with the help of anionic surfactant (sodium dodecyl benzene sulphate). The mechanism was studied and discussed in terms of the RLS and UV-absorption spectra. Under optimal experimental conditions, at 366 nm, there was a linear relationship between the RLS intensity and the concentration of the metallothioneins in the range of 0.04–14.0 μg mL⁻¹, with a correlation coefficient of r = 0.9985 and detection limit of 11.9 ng mL⁻¹. The relative standard deviation was 3.1% (n = 11), and the average recovery was 95.2%. The method proposed was reliable, selective and sensitive in determining trace metallothioneins in human urine samples with the results in good agreement with those obtained by high-performance liquid chromatography.

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The authors gratefully acknowledge the support of the Natural Science Foundation of China [No. 31200140 and 11475079], the Defense Industrial Technology Development Program [No. B3720132001], and the construct program of Innovation Team in USC [No. NHCXTD15].

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Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

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Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

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