A novel non-enzymatic amperometric H₂O₂ sensor based on screen-printed electrode modified with reduced graphene oxide, polyneutralred and gold nanoparticles

ABSTRACT

A non-enzymatic amperometric sensor for hydrogen peroxide (H₂O₂) was developed based on the screen-printed electrode (SPCE) modified with reduced graphene oxide (RGO), polyneutral red (PNR) and gold nanoparticles (AuNP). Electrochemical behaviour of hydrogen peroxide on SPCE/RGO/PNR/AuNP was investigated. The prepared sensor showed a high electrocatalytic effect on the reduction of hydrogen peroxide. The non-enzymatic sensor response for H₂O₂ was investigated as a function of pH and working potential. Optimum values of these parameters in the H₂O₂ determination were found as 7.0 for pH and −0.45 V for working potential. Amperometry was used successfully for the determination of H₂O₂ in the linear response range from 21.16 to 2730 μM with a detection limit of 6.35 μM. The relative standard deviation (RSD) was calculated to be 1.70% (for n = 10). The operational stability studies have shown that the initial amperometric response of the sensor to H₂O₂ decreased by 60.82% on the 60th day. Storage life studies have shown that the sensitivity of the sensor decreased by 40.31% at the end of 12 weeks. The non-enzymatic sensor has been tested for H₂O₂ determination in human serum sample. The developed H₂O₂ sensor is promising to be used for H₂O₂ analysis in human serum samples as simple, practical and disposable device without requiring laborious sample pre-treatment producers.

KEYWORDS:

• H₂O₂ sensor
• reduced graphene oxide
• polyneutral red
• gold nanoparticles
• screen-printed electrodes
• human serum

Disclosure statement

No potential conflict of interest was reported by the authors.

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