Assessment of bacterial toxicity of zeolitic imidazole framework-8

ABSTRACT

In this study, toxicity of zeolitic metal organic framework-8 nanoparticles on the growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus at concentrations of 5–2500 mg/L was assessed. The data were analysed using the Probit analysis and the values of no observed effect concentration (NOEC), lethal concentration-50 (LC₅₀) and 100% bacterial growth inhibition were determined. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were quantified by micro-dilution method. The 24-h LC₅₀ was 435.24, 327.08 and 76.54 mg/L and NOEC was 147.59, 103.851 and 25.662 mg/L for E. coli, P. aeruginosa and S. aureus, respectively. The 100% growth inhibitory as observed at 2009.093, 1657.094 and 359.833 mg/L, for E. coli, P. aeruginosa and S. aureus, respectively. The lowest MIC and MBC were 12.98 and 26.04 mg/L for S. aureus. This study showed that S. aureus was the suitable indicator due to its high sensitivity to zeolitic metal organic framework-8.

KEYWORDS:

• Metal organic- framework
• bacterial toxicity
• NOEC
• LC₅₀

Acknowledgments

The present article is extracted from the MSc thesis approved by Gonabad University of Medical Sciences with grant no. 557.1.p. The authors appreciate the financial support of this university. The authors also thank Dr Ahmad Zarei in Gonabad University of Medical Sciences for improving the text.

Disclosure of potential conflicts of interest

No potential conflict of interest was reported by the author(s).