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Abstract
A new sensitive spectrophotometric method for determination of pyridine at ppm level is described. The method is based on the reaction of pyridine with cyanogen bromide to form glutaconic aldehyde, which is subsequently coupled with 4-aminosalicylic acid. The yellow polymethine dye formed, which is extractable in iso-amyl alcohol, shows an absorbance maxima at 400 nm. The reaction is very sensitive and follows Beer's law in the range of 0.025 to 0.2 ppm in the extractive system. The method has been successfully applied for the determination of pyridine in waste water, benzene, laboratory air and environmental samples.
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