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Abstract
A confirmatory and quantitative technique is developed for domoic acid (DA) in seafood samples which is based on methanol-water extraction, separation by reverse phase liquid chromatography, a ninhydrin post-column derivatization of the acid, and UV detection at 402 nm. Tissue samples containing as low as 0.3 μg DA/g analyzed by a direct method using 242 nm can be confirmed by this technique. The linear range is excellent (1 ng to 4 μg) with a linear correlation coefficient of 0.994. Spike recovery of DA was 93% at a tissue concentration of 10 μg DA/g. The method was applied successfully to mussels, crabs, oysters, razor clams, and anchovies.