The inactivated infectious bronchitis virus (IBV) vaccine used as booster in layer hens influences the breadth of protection against challenge with IBV variants

ABSTRACT

To achieve long term protection of laying and breeding hens against aberrant egg production caused by infectious bronchitis virus (IBV), a vaccination programme incorporating both live-attenuated and inactivated IBV vaccines is required. High quality IBV vaccines of both types are widely available, but the number of IBV variants of global importance continues to increase and it is not possible to develop vaccines against each one of them. Therefore, it is desirable to perform studies under controlled conditions to determine which IBV vaccine(s) provide the best protection for laying hens against different IBV challenges. Previous vaccination and challenge studies have shown that it is possible to obtain relevant data in a small number of laying hens housed under conditions of strict isolation. The present work extends this finding by investigating the efficacy, against challenge with five IBV strains of global importance, of an IBV vaccination programme including two live-attenuated IBV vaccines (Massachusetts and 793B types) and three different commercially available inactivated vaccines each containing antigen against at least one IBV strain. The results reported here confirm the importance of IBV vaccination for laying hens, show that efficient live priming makes a beneficial contribution to this protection and confirm that inactivated IBV vaccines contribute significantly to effective protection against at least the five IBV challenge strains used here. Furthermore, we provide data to support the “protectotype concept”, long-established using different live-attenuated IBV vaccines in young chickens, is valid in broadening protection against IBV challenges in laying birds.

RESEARCH HIGHLIGHTS

• IBV vaccination is essential as an aid in protecting laying hens against IBV infection.

• Live priming is a beneficial part of the IBV vaccination programme.

• IBV inactivated vaccine improves IBV protection.

• Heterologous IBV protection is confirmed in laying hens.

KEYWORDS:

• Infectious bronchitis virus
• IBV variants
• IBV protection in layers
• IBV vaccination
• drops in egg production
• IBV challenge
• protectotype

Acknowledgements

The authors would like to express their gratitude to the animal caretakers of the Royal GD for their excellent technical assistance.

Disclosure statement

No potential conflict of interest was reported by the authors.