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Abstract
A systematic approach to the chemical synthesis of oligoribo- and oligodeoxyribo-nucleotides is described. Synthesis in both series of oligonucleotides has been tackled as a two-stage problem. The first stage, which is concerned with the preparation of correctly-orientated, suitably-protected nucleoside building blocks, has been completed. The second stage, which is concerned with the development of an effective phosphorylation procedure for linking the building blocks together, is presently being investigated. From the results obtained so far, it is apparent that the phosphotriester approach to the synthesis of oligonucleotides, especially with phenyl (and other aryl) protecting groups, is superior to approaches which involve unprotected internucleotide linkages during synthesis.