63
Views
5
CrossRef citations to date
0
Altmetric
Original

Mathematical model of an amperometric biosensor for the design of an appropriate instrumentation system

&
Pages 351-360 | Published online: 09 Jul 2009
 

Abstract

In this paper, a mathematical model for a membrane based amperometric biosensor is developed. The model is based on a diffusion mechanism related to Michaelis-Menten kinetics. The model is developed for an intensive stirred condition, so it has been assumed that the thickness of the diffusion layer is negligible. The model can be used to investigate the regularities and kinetics of the amperometric biosensor, and to develop any simulation methods to study the biosensor. The model shows that current I(t) generated during the specific biosensor enzymatic reaction mainly depends on the number of electrons generated and the area of working electrode. The model also describes the effect of background current in the biosensor. The validity of the developed model has been verified by designing a computer based instrumentation system for the amperometric biosensor. Repeated real time experiments were carried out, and the results obtained are in excellent agreement with the amount determined by high performance liquid chromatographic technique (HPLC), with an accuracy of ±1.5%.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.