Detection of arbuscular mycorrhizal fungi within colonised roots of the Gramineae family members by nested-polymerase chain reaction (PCR)

Abstract

In this study, it is aimed to asses the association of arbuscular mycorrhizal (AM) fungi within colonised rhizosphere of Gramineae family members through a survey by using nested- polymerase chain reaction method in Van province (Turkey). From 24 agro-ecological fields, a total of 82 samples belonging to Gramineae family were tested by molecular methods. The presence of Glomus intraradices and Glomus mosseae was ascertained in 10 plants belonging to eight different species by using fungus specific primers. Root colonisation ranged from 6 to 37% within rhizosphere of Gramineae family members and the average root colonisation by AM fungi was 22%.

Keywords:

• Gramineae
• arbuscular mycorrhizal fungi
• nested-PCR

Acknowledgement

Financial support for this study was provided by The Scientific and Technical Research Council of Turkey, TUBITAK (Project no: TOGTAG-3367).