Abstract
Root-knot nematode, Meloidogyne javanica, is a major problem confronting greenhouse’s productions, field crops, vegetables, grapevines and almond rootstocks in Kermanshah province, Iran. Nematicides are not affordable to control this nematode. In the search for alternatives to chemicals control of nematodes, this research has dealt with nematicidal effects of crude herbal extracts on the root-knot nematodes. This study aimed to evaluate the effect of 21 endemic and exotic herbal extracts belong to 12 families of flowering plants in comparison with chicken manure and chemical nematicide (Temik) to control root-knot nematodes in in vitro conditions. The nematodes were pured and mass multiplied on tomato in the soil at greenhouse conditions. In order to study the effect of herbal extracts on mortality of second-stage juveniles (J2), a 6 mL of each extract was poured in sterilised Petri dish and 54 ± 4 juveniles were added. Distilled water was used as control and treatments replicated four times and incubated at ambient temperature. The LC50 value of each extract was determined by assessing the mortality of juveniles (in the range of 5–95%) after 24, 48 and 72 h. Comparison between LC50 value of the extracts indicated that Cinnamomum zeylanicum and Eugenia caryophillata are the most effective crude extracts on the mortality of juveniles and they were 15.4 and 17.9 mg mL−1, respectively. Meanwhile, the extract of tobacco, ferulago, garlic, eucalyptus, persan lilac, rattle, oliveria, licorice, russian knapweed, turnsole, sicilian sumac and chicken manure did not have any antinematode activity against fresh second-stage juveniles of the root-knot nematode.
Acknowledgements
We wish to express our sincere thanks to Dr Saeed Abbasi, member of plant pathology, Dept. of College of Agriculture, Razi University, for providing technical assistance.