Application of Bacillus pumilus as a potential biocontrol agent of Fusarium wilt of tomato

Abstract

The current study aimed at evaluating the possibility of native Bacillus pumilus species to control Fusarium wilt in tomato and examine its effect on plant growth. Biocontrol traits of B. pumilus strains, biofilm assay, root colonisation and in vivo studies under pot conditions were determined. Strain ToIrMA-KC806242 formed biofilm efficiently and could colonise and survive on tomato rhizosphere (3.1 × 10⁴ CFU/g of root). The amount of auxin production was recorded 29.7 μg/ml at the 96th hour of incubation. Siderophore production was determined positive, while ToIrMA was not able to solubilise phosphate compounds or produce cyanide hydrogen. Statistical analysis of data revealed that the increase in root and shoot length was recorded 60 and 84%, respectively, over control. In addition, about 73% reduction in disease incidence was determined in vivo experiments. In conclusion, this study suggests B. pumilus ToIrMA strain as a possible biocontrol agent in the field experiments.

Keywords:

• Bacillus pumilus
• biocontrol
• Fusarium wilt
• indigenous

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study is supported by the Research Council of Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.