Abstract
The fungitoxic effect of Moringa oleifera extracts (MOE) were assessed in vitro and in vivo against Colletotrichum gloesporioides, a causal fungal pathogen of anthracnose in mango fruits. In vitro test result showed that MOE at 2.25 to 3.00 g/L concentration completely inhibits the growth of C. gloesporioides. Spore shredding, cytoplasmic discharge and mycelial blast were vividly observed when C. gloesporioides was subjected with MOE treatment. The minimum effective concentration (MEC) of MOE at 2.25 g/L can be applied as curative, protective and simultaneous treatment on mango fruit to inhibit the anthracnose infection. The efficacy of MOE was relatively superior to synthetic fungicide based on protective, curative and simultaneous treatments. Forty-three chemical components were identified in Philippine native Moringa, based on GCMS analysis. The seven major compounds were as follows: oleyl alcohol, methyl ether (18.16%), γ-sitosterol (7.29%), 3-heptanol,6-methyl (6.88%), linalool oxide (4.95%), benzyl-o-β-d-glucoside (4.83%), formic acid.2-methyl-(1.3)dithian-2-yl methyl ester (3.51%), and nonadecanoic acid (3.15%).
Acknowledgements
The authors acknowledge Prof. Young Soo Keum, Prof. Doo Hwan Kim, Se Chul Chun of Konkuk University (KU) Seoul, South Korea, the Korea International Cooperation Agency (KOICA), Davao Analytical Laboratories headed by Sir Tony Gumapac, Dr. Jay Ronel Cornejos, Dr. Brian Lopez for the assistance.
Disclosure statement
The authors declare that there are no conflicts of interest.