Morphological, phylogenetic, and pathogenic analyses of Fusarium andiyazi associated with sugar beet root diseases

Abstract

Fusarium andiyazi isolates, EGF1 and EGF2, were recovered from sugar beets exhibiting wilting, chlorosis, and root rot. Morphological characteristics and sequencing of translation elongation factor-1α (TEF-1α), the ITS region of rDNA, and β-tubulin (TUB) wear combined to corroborate the identity of these isolates. Phylogenetic analysis of the partial TEF gene revealed genetic variation among F. andiyazi isolates in three genetic clades. Both isolates infected seedlings of all sugar beet cultivars and caused varying degrees of wilting symptoms. 38-day-old sugar beet cultivars differed in their response to the isolates, being either susceptible (Kawemira) or resistant (Pleno). Both isolates induced symptoms of wilting and root rot in sorghum cultivars and caused bakanae in rice cultivars. Cultivars of sorghum (Dorado) and rice (Sakha 101) were susceptible to F. andiyazi infection. This is the first report of F. andiyazi, isolated from sugar beets, causing typical symptoms of seedling wilt and root rot.

Keywords:

• Fusarium andiyazi
• sugar beet
• root rot
• TEF-1α and ITS-rDNA

Disclosure statement

No potential conflict of interest was reported by the author(s).