https://orcid.org/0000-0002-8441-5769
![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
ABSTRACT
This study aimed to find out the optimal formulation of essential oil-encapsulated lipid nanoemulsions (EO-LNs) by comparing antifungal activity against leaf spot disease caused by Alternaria alternata on tomato plants of 10 different EO-LNs, followed by their binary and ternary combinations. Firstly, 10 EO-LNs and their combined formulations were prepared and their in-vitro antifungal activity was evaluated using petri dish bioassay. Next, ex-vitro disease-prevention and disease-treatment experiments of the optimal EO-LNs formulation were carried out in the greenhouse. The results indicated that there was a significant difference in in-vitro antifungal activity against Alternaria alternata among 10 EO-LNs and their combined formulations. Overall, NaChiGarCin (the ternary combination of chili oil-encapsulated LNs, garlic oil-encapsulated LNs, cinnamon oil-encapsulated LNs) exhibited the highest in-vitro antifungal activity. Under ex-vitro condition, NaChiGarCin at an EO-concentration of 200 ppm showed the highest disease-preventing and treating effectiveness (above 80%) and showed no negative effect on the plant growth.
Supplemental data for this article is available online at http://dx.doi.org/10.1080/03235408.2021.2015887 .
Disclosure statement
No potential conflict of interest was reported by the authors.
Funding
This study was partly supported by Institute of Developmental Philosophy (Vietnam) and Nuclear Research Institute (Vietnam).
Authors’ contributions
MHN contributed to the conception, design of the article. The experiments were carried out by MHN, NBDV and TNMT. Data curation and analysis were done by MHN. Writing and revising the entire manuscript was done by MHN, NBDV and TNMT. The authors have read, revised and approved the manuscript.